Combination Therapy of Oncolytic Virus and CAR-NK Cells in Glioma Mouse Model

6–8-week-old male C57BL/6 mice were implanted with the murine CT2A cell line expressing human EGFR (CT2A-hEGFR). Five days after the CT2A-hEGFR cells were implanted, mice were treated with 2 × 10⁵ pfu OV-IL15C alone, 1 × 10⁶ frozen and unsorted EGFR-CAR NK cells alone, the combination of the two agents, or saline alone. Three days after the treatment, mice were sacrificed to harvest brain tissues to isolate mononuclear cells, as previously described (22 (link),23 (link)). The mononuclear cells were subjected to assess NK and T cell infiltration or cultured with PMA (BioLegend) and 1 mg/ml of GolgiPlug^™ for 4 hours before assessing the activation capacity of these cytolytic cells by flow cytometry.