Adenoviruses expressing a TSC22D4- or LCN13- or a non-specific shRNA under the control of the U6 promoter, or the TSC22D4 cDNA under the control of the CMV promoter were cloned using the BLOCK-iT Adenoviral RNAi expression system (Invitrogen). Viruses were purified by the cesium chloride method and dialysed against phosphate-buffered-saline buffer containing 10% glycerol before animal injection, as described23 (link). Adeno-associated viruses encoding control or TSC22D4-specific miRNAs under the control of a hepatocyte-specific promoter were established, as described previously22 (link).
Ekim Üstünel B., Friedrich K., Maida A., Wang X., Krones-Herzig A., Seibert O., Sommerfeld A., Jones A., Sijmonsma T.P., Sticht C., Gretz N., Fleming T., Nawroth P.P., Stremmel W., Rose A.J., Berriel-Diaz M., Blüher M, & Herzig S. (2016). Control of diabetic hyperglycaemia and insulin resistance through TSC22D4. Nature Communications, 7, 13267.
Other organizations :
German Center for Diabetes Research, University Medical Centre Mannheim, Heidelberg University, University Hospital Heidelberg, Leipzig University
Adenoviruses expressing a TSC22D4- or LCN13- or a non-specific shRNA under the control of the U6 promoter
Adeno-associated viruses encoding control or TSC22D4-specific miRNAs under the control of a hepatocyte-specific promoter
dependent variables
Not explicitly mentioned
control variables
Phosphate-buffered-saline buffer containing 10% glycerol used for dialysis before animal injection
controls
Positive control: Adenoviruses expressing a non-specific shRNA
Negative control: Adeno-associated viruses encoding control miRNAs
Annotations
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