Zebrafish Transgenic Lines for Vascular Development

All zebrafish husbandry was performed under standard conditions in accordance with institutional (UCSF and MPG) and national ethical and animal welfare guidelines. Tg(kdrl:EGFP)^s843 (Jin et al., 2005 (link)), Tg(kdrl:NLS-EGFP)^ubs1 (Blum et al., 2008 (link)), Tg(kdrl:Has.HRAS-mcherry)^s896 (Chi et al., 2008 (link)), Tg(fli1a:EGFP)^y1 (Lawson and Weinstein, 2002 (link)), TgBAC(etv2:EGFP)^ci1 (Proulx et al., 2010 (link)), Tg(-0.8flt1:tdTomato)^hu5333 (Bussmann et al., 2010 (link)), TgBAC(flt1:YFP)^hu4624 (Hogan et al., 2009a (link)), Tg(elavl3:gal4-vp16)^psi1 (Stevenson et al., 2012 (link)), TgBAC(gfap:gfap-gfp)^zf167 (Lam et al., 2009 (link)), Tg(gfap:GFP)^mi2001 (Bernardos and Raymond, 2006 (link)), Tg(elavl3:EGFP)^knu3 (Park et al., 2000 (link)), Tg(UAS-E1b:NfsB-mCherry)^c264 (Davison et al., 2007 (link)), Tg(mpx:gal4)^sh267 (Robertson et al., 2014 (link)), Tg(-4.9sox10:EGFP)^ba2(Carney et al., 2006 (link)), Tg(hsp70l:nog3)^fr14 (Chocron et al., 2007 (link)), irf8^st96 (Shiau et al., 2015 (link)), pdgfrb^um148 (Kok et al., 2015 (link)), erbb2^st61 (Lyons et al., 2005 (link)), and flt1^fh390 (Rossi et al., 2016 (link)) were used in this study. Fish embryos/larvae were raised at 28°C.

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Matsuoka R.L., Marass M., Avdesh A., Helker C.S., Maischein H.M., Grosse A.S., Kaur H., Lawson N.D., Herzog W, & Stainier D.Y. (2016). Radial glia regulate vascular patterning around the developing spinal cord. eLife, 5, e20253.

Publication 2016

Embryos Fish Flt1 Gal4 vp16 Gfap Hras Larvae Tdtomato Zebrafish

Corresponding Organization :

Other organizations : Max Planck Institute for Heart and Lung Research, University of Massachusetts Chan Medical School, Max Planck Institute for Molecular Biomedicine

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Protocol cited in 12 other protocols

Variable analysis

independent variables

Tg(kdrl:EGFP)s843
Tg(kdrl:NLS-EGFP)ubs1
Tg(kdrl:Has.HRAS-mcherry)s896
Tg(fli1a:EGFP)y1
TgBAC(etv2:EGFP)ci1
Tg(-0.8flt1:tdTomato)hu5333
TgBAC(flt1:YFP)hu4624
Tg(elavl3:gal4-vp16)psi1
TgBAC(gfap:gfap-gfp)zf167
Tg(gfap:GFP)mi2001
Tg(elavl3:EGFP)knu3
Tg(UAS-E1b:NfsB-mCherry)c264
Tg(mpx:gal4)sh267
Tg(-4.9sox10:EGFP)ba2
Tg(hsp70l:nog3)fr14
Irf8st96
Pdgfrb um148
Erbb2st61
Flt1fh390

dependent variables

Not explicitly mentioned

control variables

All zebrafish husbandry was performed under standard conditions in accordance with institutional (UCSF and MPG) and national ethical and animal welfare guidelines.
Fish embryos/larvae were raised at 28°C.

positive controls

Not specified

negative controls

Not specified

Annotations

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