Whole retina was carefully isolated from the paraformaldehyde fixed eye, and incubated at 37°C for 45 to 75 minutes in 3% crude trypsin (Gibco, Grand Island, NY, USA) solution containing 200 mM sodium fluoride. The neuroretinal tissue was gently brushed away under a microscope, and the vasculature was stained with periodic acid Schiff–hematoxylin to count the acellular capillaries by light microscopy, and imaged using the Zeiss ApoTome microscope using a 40× objective.3 (link),20 (link)
Protocol detail
Retinal Vasculature Isolation and Quantification
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Capillaries
Light microscopy
Microscope
Paraformaldehyde
Periodic acid
Retina
Sodium fluoride
Tissue
Trypsin
Corresponding Organization : Wayne State University
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Duration of incubation at 37°C (45 to 75 minutes)
- Number of acellular capillaries
- Whole retina carefully isolated from the paraformaldehyde fixed eye
- 3% crude trypsin (Gibco, Grand Island, NY, USA) solution containing 200 mM sodium fluoride
- Periodic acid Schiff–hematoxylin staining of the vasculature
- Imaging using the Zeiss ApoTome microscope with a 40× objective
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!