Immuoblots and BrdU immunofluorescence assays were carried out as described previously [13 (link),14 (link)]. The following antibodies (all from Santa Cruz Biotechnology) were used: anti-p21 antibody (clones M-19, C-19 and F-5), anti-TRIM3 antibody (clone 27), anti-c-Myc antibody (clone 9E10), anti-HA (haemagglutinin) antibody (clone Y-11), anti-tubulin antibody, anti-GST antibody (clone B-14), anti-BSA antibody (clone A23-A/D3) and anti-p27 antibody (clone C-19).
Protocol detail
Immunoblotting and BrdU Immunofluorescence Assays
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Anti antibody
Anti c antibody
Antibodies
Antibody
Brdu
Clone
Haemagglutinin
Immunofluorescence assays
Tubulin
Corresponding Organization :
Other organizations : Cornell University, Memorial Sloan Kettering Cancer Center
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Variable analysis
independent variables
- Immunoblots
- BrdU immunofluorescence assays
- Protein expression levels (p21, TRIM3, c-Myc, HA, tubulin, GST, BSA, p27)
- Antibodies (anti-p21, anti-TRIM3, anti-c-Myc, anti-HA, anti-tubulin, anti-GST, anti-BSA, anti-p27)
- Not explicitly mentioned
- Not explicitly mentioned
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