Whole genome chromosome conformation capture (Hi-C) was performed as in [96 (link)] with restriction enzyme MboI (NEB) [96 (link)]. Formaldehyde fixed cells from 4 to 5 migration assays were pooled to reach 2 million cells required. The library was prepared for Illumina sequencing with the TruSeq Nano kit (Truseq Nano DNA LT Sample Preparation Kit). One library was sequenced per lane of the Illumina HiSeq X 150PE.
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