The paraffin-embedded nucleus pulposus tissues were processed for immunohistochemical staining as previously described.33 (link) The primary antibody used was caspase-1 (1:150, Proteintech, Chicago, USA, Cat. No.: 22915-1-AP) and the secondary antibody was goat anti-rabbit IgG (H+L) HRP (1:200, Affinity Biosciences, USA, Cat. #: S0001). The sections were visualized using a microscope (CTR4000B, Leica). Experiments were repeated three times independently.
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Immunohistochemical Analysis of Caspase-1 in Nucleus Pulposus
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Other organizations : Wenzhou Medical University, First Affiliated Hospital of Wenzhou Medical University, Zhejiang Hospital
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- Primary antibody used was caspase-1 (1:150, Proteintech, Chicago, USA, Cat. No.: 22915-1-AP)
- Immunohistochemical staining of paraffin-embedded nucleus pulposus tissues
- Secondary antibody was goat anti-rabbit IgG (H+L) HRP (1:200, Affinity Biosciences, USA, Cat. #: S0001)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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