Immune Cell Profiling in Tumors

The frequency of different immune cell types was determined using FACS. Myeloid-derived suppressor cells (MDSCs) in tumor or spleen tissue were analyzed using a mouse MDSC Flow Cocktail 1 (CD11b PE/Gr-1 APC/Ly-6G FITC, BioLegend). Regulatory T cells were analyzed using the Mouse Regulatory T Cell Staining Kit #1 (eBioscience). FITC anti-mouse CD8α antibody (clone: 53-6.7, BioLegend), APC anti-mouse CCR5 antibody (clone: HM-CCR5, BioLegend), PE anti-mouse CCR6 antibody (clone: 29-2L17, BioLegend), and PE/Cy7 anti-mouse CCR7 antibody (clone: 4B12, BioLegend) were used to label isolated CD11c⁺ cells (38 (link)).

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Liu C., Cong X., Wang Y., Guo Q., Xie Y., Geng F., Guo J., Dong L., Zhou Y., Wu H., Yu B., Wu J., Zhang H., Yu X, & Kong W. (2021). Fast DNA Vaccination Strategy Elicits a Stronger Immune Response Dependent on CD8+CD11c+ Cell Accumulation. Frontiers in Oncology, 11, 752444.

Publication 2021

Mouse Regulatory T Cell Staining Kit #1

Anti antibody Ccr5 Ccr6 Cd11b Cells Clone Fitc Mdsc Mouse Regulatory t cell Spleen Tissue Tumor

Corresponding Organization : Jilin University

Other organizations : Union Hospital

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Variable analysis

independent variables

Myeloid-derived suppressor cells (MDSCs) in tumor or spleen tissue
Regulatory T cells
Isolated CD11c+ cells

dependent variables

Frequency of different immune cell types

control variables

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

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