Immunohistochemistry and Immunofluorescence of Alzheimer's Pathologies

IHC and IF were performed as previously described^{12 (link),14 (link)}. For IHC staining of NP-tau and Aβ, primary antibodies AT8 (Thermo Fisher Scientific, MN1020B, 1:500) and HJ3.4 (2 μg/ml in-house) respectively were used.
For IF staining, co-stains were performed for X34, BACE1, and AT8. Fibrillar Aβ was stained with X34 dye (SML-1954) and antibodies to AT8 and BACE1 (abcam, ab108394, 1:500) were used to evaluate peri-plaque pathologies.

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Gratuze M., Jiang H., Wang C., Xiong M., Bao X, & Holtzman D.M. (2022). APOE immunotherapy mitigates cerebral Aβ accumulation and Aβ-associated tau seeding and spreading in a mouse model of amyloidosis. Annals of neurology, 91(6), 847-852.

Publication 2022

MN1020B ab108394

Antibodies Bace1 Plaque Stains

Corresponding Organization : Hope Center for Neurological Disorders

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Variable analysis

independent variables

Primary antibodies AT8 and HJ3.4 used for IHC staining of NP-tau and Aβ, respectively
Co-stains performed for X34, BACE1, and AT8 for IF staining

dependent variables

NP-tau and Aβ staining using IHC
Fibrillar Aβ, peri-plaque pathologies, and BACE1 expression using IF staining

control variables

IHC and IF were performed as previously described in the cited references (12 and 14)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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