Standardized Preparation and Composition of HZOL

HZOL was purchased from the Taiji Group Chongqing Fuling Pharmaceutical Co., Ltd. Production of HZOL following the Chinese Pharmacopeia 2015 (Approved number, Z50020409; Lot number, 20071956). Quality of HZOL was able to ascertain both stability and controllability. Introduction of HZOL was follows: firstly, prescription of HZOL consists of ten herbs including Atractylodis Rhizoma 80 g, Magnoliae Officinalis Cortex 80 g, Poria 120 g, Pinelliae Rhizoma 80 g, Citri Reticulatae Pericarpium 80 g, Angelicae Dahuricae Radix 120 g, Arecae Pericarpium 120 g, Licorice extract 10 g, Patchouli oil 0.8 mL, and volatile oil in Perillae Folium 0.4 mL. Secondly, HZOL was standardised prepared following the method of the criteria of the Chinese Pharmacopeia 2015 as previous study described [10 (link)]. Thirdly, content determination of HZOL was controlled with magnolol, honokiol, and hesperidin. In a nutshell, per milliliter of HZOL contained magnolol and honokiol no less than 0.30 mg and hesperidin no less than 0.10 mg.