Antibody-Loaded Polymeric Nanoparticles

Dodecyl-modified (hydroxypropyl)methyl cellulose (HPMC-C₁₂, made from USP-grade HPMC (hypromellose, Sigma-Aldrich)) and poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) block copolymers were synthesized as previously described; PEG-b-PLA nanoparticles (NPs) were assembled by nanoprecipitation as previously described.^{27,28,42 (link)} Unless otherwise stated, all chemicals were obtained from Sigma-Aldrich. Purified rat IgG was obtained from MP Biomedicals as a lyophilized powder. The Centi-C10 antibody against SARS-CoV-2 was generously provided by Dr. Sawsan Youssef and Dr. Jacob E. Glanville at Centivax.

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Kasse C.M., Yu A.C., Powell A.E., Roth G.A., Liong C.S., Jons C.K., Buahin A., Maikawa C.L., Zhou X., Youssef S., Glanville J.E, & Appel E.A. (2023). Subcutaneous delivery of an antibody against SARS-CoV-2 from a supramolecular hydrogel depot. Biomaterials Science, 11(6), 2065-2079.

Publication 2023

PEG-b-PLA Purified rat IgG

Antibody Block Hypromellose Peg pla Poly ethylene glycol Poly lactic acid Powder Sars cov 2

Corresponding Organization : Stanford University

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Variable analysis

independent variables

Dodecyl-modified (hydroxypropyl)methyl cellulose (HPMC-C₁₂)
Poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) block copolymers

dependent variables

PEG-b-PLA nanoparticles (NPs)

control variables

Purified rat IgG
Centi-C10 antibody against SARS-CoV-2

notes

The researchers synthesized the HPMC-C₁₂ and PEG-b-PLA block copolymers, which are the independent variables manipulated in the study.
The formation of PEG-b-PLA nanoparticles (NPs) is the measured outcome, which is the dependent variable.
The purified rat IgG and Centi-C10 antibody against SARS-CoV-2 are used as controls in the experiment.

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