Histological Analysis of Liver Tissue

Hematoxylin and eosin (HE) staining was performed on liver tissue using standard protocols previously described by Cui et al. (2020) and Amevor et al. (2021) (link). The tissues (µm/g) were fixed for 24 h and embedded in paraffin. Thereafter, tissue sections were selected for histological and morphological observations. Frozen sections of the liver tissue were carbowax-embedded, oil-red staining was performed, and then all the sections were viewed under a fluorescence microscope and photos were taken (DP80 Digital, Olympus, Tokyo, Japan).

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Amevor F.K., Cui Z., Ning Z., Shu G., Du X., Jin N., Deng X., Xu D., Tian Y., Zhang Y., Li D., Wang Y., Du X, & Zhao X. (2022). Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens. Poultry Science, 101(6), 101851.

Publication 2022

DP80 Digital

Carbowax Digital Embedded paraffin Eosin Fluorescence microscope Frozen sections Hematoxylin Liver Tissue

Corresponding Organization : Sichuan Agricultural University

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Variable analysis

independent variables

Liver tissue preparation method (HE staining, oil-red staining)

dependent variables

Histological and morphological observations of liver tissue

control variables

Tissue fixation time (24 h)
Paraffin embedding of tissue
Tissue sectioning
Fluorescence microscope used for imaging

controls

Standard protocols previously described by Cui et al. (2020) and Amevor et al. (2021) for HE staining

Annotations

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