Enzymatic Starch Hydrolysis Protocol

Sodium molybdate dihydrate (Na₂MoO₄x2H₂O), sodium sulfate (Na₂SO₄), sodium carbonate (Na₂CO₃), sodium bicarbonate (NaHCO₃), sodium-potassium tartrate tetrahydrate (KOCOCH(OH)CH(OH)COONax4H₂O), copper sulfate pentahydrate (CuSO₄x5H₂O), potassium iodide (KI), iodine (I₂), hydrochloric acid (HCl), and glucose were all purchased from POCh (Gliwice, Poland). Sodium arsenate dibasic heptahydrate (Na₂HAsO₄x7H₂O) and rice starch were purchased from Sigma (St. Louis, USA). The purified enzymatic preparation of the recombinant insect alpha-amylase (SoAMY), as well as the recombinant fungal glucoamylase (TlGAMY), was obtained and purified as described previously (Celińska et al. 2015 (link), 2018 (link)).

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Borkowska M., Białas W., Kubiak M, & Celińska E. (2019). Rapid micro-assays for amylolytic activities determination: customization and validation of the tests. Applied Microbiology and Biotechnology, 103(5), 2367-2379.

Publication 2019

Sodium arsenate dibasic heptahydrate rice starch

Alpha amylase Copper sulfate Enzymatic Glucoamylase Glucose Hydrochloric acid Insect Nahco3 Potassium iodide Rice Sodium arsenate Sodium carbonate Sodium molybdate Sodium potassium tartrate Sodium sulfate Starch

Corresponding Organization : University of Life Sciences in Poznań

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Variable analysis

independent variables

Sodium molybdate dihydrate (Na2MoO4x2H2O)
Sodium sulfate (Na2SO4)
Sodium carbonate (Na2CO3)
Sodium bicarbonate (NaHCO3)
Sodium-potassium tartrate tetrahydrate (KOCOCH(OH)CH(OH)COONax4H2O)
Copper sulfate pentahydrate (CuSO4x5H2O)
Potassium iodide (KI)
Iodine (I2)
Hydrochloric acid (HCl)
Glucose
Sodium arsenate dibasic heptahydrate (Na2HAsO4x7H2O)
Rice starch
Recombinant insect alpha-amylase (SoAMY)
Recombinant fungal glucoamylase (TlGAMY)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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