The wild-type (K3) line of hiPSC was established previously by Si-Tayeb et al. [27 (link)] and was provided for our studies. The cell line was maintained in StemFlex medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) on Vitronectin-coated dishes at 37 °C with 5% CO2. The culture medium was replaced every 2 days and hiPSCs were passaged with Accutase (Thermo Fisher) every 4–6 days. A 10 μM quantity of Y-27632 ROCK inhibitor (Tocris) was added to the culture media for 24 h after each passage. Cell passages between No. 50 and No. 60 were used for the experiments.
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