Molecular Cloning and Manipulation of ZEB1 and ATM

The human complementary DNAs (cDNAs) fragment encoding the full-length ZEB1 sequence^{41 (link)} was prepared by PCR and cloned into pLV-EF1-MCS-IRES-Bsd (Biosettia). The lentiviral-based vector pLV-H1-EF1α-puro (Biosettia) was used to express shRNAs in breast cancer cells. The human ATM promoter (−1534/+235) sequences were obtained by PCR from human genomic DNA and cloned into pGL3‑basic vector (Promega). Mutagenesis of the E₂‑box in the human ATM promoter was performed using a QuikChange Site‑Directed Mutagenesis kit (Stratagene).

Free full text: Click here

Zhang X., Zhang Z., Zhang Q., Zhang Q., Sun P., Xiang R., Ren G, & Yang S. (2018). ZEB1 confers chemotherapeutic resistance to breast cancer by activating ATM. Cell Death & Disease, 9(2), 57.

Publication 2018

pLV-EF1-MCS-IRES-Bsd pLV-H1-EF1α-puro pGL3‑basic vector

Breast cancer Cdnas Cells Human Human genomic Ires Mutagenesis Pgl3 Promega Shrnas Site directed mutagenesis Vector

Corresponding Organization :

Other organizations : First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Nankai University, Tianjin First Center Hospital, Tianjin Medical University, Wake Forest University

Top 5 similar protocols

Variable analysis

independent variables

Lentiviral-based vector pLV-H1-EF1α-puro to express shRNAs in breast cancer cells
Mutagenesis of the E2-box in the human ATM promoter

dependent variables

Expression of ZEB1 sequence
Expression of ATM promoter activity

control variables

Human complementary DNAs (cDNAs) fragment encoding the full-length ZEB1 sequence
Human ATM promoter (-1534/+235) sequences
PGL3-basic vector

positive controls

None specified

negative controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!