All crosses and staging were performed at 25°C unless otherwise noted. w1118 was used as wild-type. Stocks are described in FlyBase (http://flybase.org/). pucE69, UAS-P35 (B#5073), UAS-Scrib-RNAi (B#29552), UAS-Bsk-RNAi (B#32977), UAS-Moesin-myc (B#8631), UAS-Moesin-RNAi (B#8629), UAS-Mekk1-RNAi (B#28587), UAS-Ask1-RNAi (B#32464), UAS-Hep-RNAi (B#28710), UAS-Mkk4-RNAi (B#35140), UAS-p38b-RNAi (B#35252), UAS-p38a-RNAi (B#34744), bsk1 (B#3088), UAS-Baz-RNAi (B#39072), UAS-aPKC-RNAi (B#25946) and “Dlg-RNAi #2” (B#35286) were provided by the Bloomington Drosophila Stock Center (BDSC); Zip1 by T. Wolff (NIH Janelia Research Campus); patched-GAL4 by R. Cagan (Mount Sinai School of Medicine, New York); UAS-Cdc42- RNAi and UAS-Rho1-RNAi were described previously [11 (link),12 (link)]; “UAS-Dlg-RNAi #1”(V#41134), UAS-Rok-RNAi (V#3793), and UAS-Wnd-RNAi (V#103410) were provided by the Vienna Drosophila RNAi Center (VDRC); UAS-Slpr-RNAi, UAS-Wengen-RNAi, and UAS-Tak1-RNAi were provided by R. Fehon (University of Chicago); UAS-Egr was provided by M. Vidal (Beatson Institute, Glasgow, UK). MARCM clones were generated by heat shock at 37C for 1h and dissected 40 to 48 hours after clone induction (ACI).
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