Bioactive Compound Extraction and Characterization from Stem Bark of S. pinnata

Different compounds were isolated following the method of Chaudhuri, et al. [9 (link)]. Briefly, the S. pinnata stem bark was cut into small pieces, dried, ground into powder, and extracted with 70 % methanol and water. The lyophilized extract was re-extracted successively with hexane, chloroform, ethyl acetate and water. All of the fractions were concentrated in reduced pressure, and the ethyl acetate fraction was further purified through silica gel column chromatography. Dichloromethane and methanol elution yielded four compounds namely SPE1, SPE2, SPE3 and SPE4. Structures of the bioactive compounds were analysed using different spectroscopic methods such as EIMS (JEOL JMS-700, Germany), FT-IR spectra recorded in KBr (Perkin Elmer, USA) and different nuclear magnetic resonance (NMR) experiments including ¹H and ¹³C with a Bruker-500 MHz NMR Spectrometer (Germany).