Intestinal Histomorphometry of Fish Samples

Samples of the anterior portion of the intestines of nine fish per treatment were collected and submerged in Bouin’s solution (Merck) until analysis, which was performed according to methods described by Sobolewska et al. (2017) [47 (link)]. The samples were dehydrated, cleared and embedded in paraffin blocks. Formed blocks were cut on a rotary microtome (Thermo Shandon, Chadwick Road, Astmoor, Runcorn, Cheshire, United Kingdom) into slices of 10-μm thickness [48 (link)]. The slices were placed on microscope slides coated with ovo albumin with an addition of glycerol. The samples were analyzed using an AnMN-800 F microscope (OPTA-TECH, Warsaw, Poland) equipped with an Opta-View camera for recording microscopic images. MultiScan v. 18.03 microscope imaging software (Computer Scanning Systems II Ltd., Warsaw, Poland) was used to measure villus height and width and muscular thickness.

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Mikołajczak Z., Rawski M., Mazurkiewicz J., Kierończyk B, & Józefiak D. (2020). The Effect of Hydrolyzed Insect Meals in Sea Trout Fingerling (Salmo trutta m. trutta) Diets on Growth Performance, Microbiota and Biochemical Blood Parameters. Animals : an Open Access Journal from MDPI, 10(6), 1031.

Publication 2020

Bouin’s solution

Albumin Embedded paraffin Fish Glycerol Intestines Microscope Microtome Muscular

Corresponding Organization : University of Life Sciences in Poznań

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Variable analysis

independent variables

Treatment

dependent variables

Villus height
Villus width
Muscular thickness

control variables

Anterior portion of the intestines of nine fish per treatment
Samples submerged in Bouin's solution (Merck) until analysis
Samples dehydrated, cleared and embedded in paraffin blocks
Blocks cut into 10-μm thick slices
Slices placed on microscope slides coated with ovo albumin with an addition of glycerol
Samples analyzed using an AnMN-800 F microscope (OPTA-TECH, Warsaw, Poland) equipped with an Opta-View camera
MultiScan v. 18.03 microscope imaging software (Computer Scanning Systems II Ltd., Warsaw, Poland) used to measure villus height and width and muscular thickness

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