DNA/RNA Extraction and PCR Protocols

Total cellular or tissue DNA/RNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) for DNA or the RNeasy Plus Universal Kit (Qiagen) for RNA as per the manufacturer’s instructions, as previously described (111 (link)). PCR was performed with the Platinum Taq DNA Polymerase High Fidelity Kit (Invitrogen), whereas qPCR was performed with the TaqMan RNA-to-Ct 1-Step Kit (Applied Biosystems). All reagents are listed in Table 1.

Free full text: Click here

Vasavda C., Xu R., Liew J., Kothari R., Dhindsa R.S., Semenza E.R., Paul B.D., Green D.P., Sabbagh M.F., Shin J.Y., Yang W., Snowman A.M., Albacarys L.K., Moghekar A., Pardo-Villamizar C.A., Luciano M., Huang J., Bettegowda C., Kwatra S.G., Dong X., Lim M, & Snyder S.H. (2022). Identification of the NRF2 transcriptional network as a therapeutic target for trigeminal neuropathic pain. Science Advances, 8(31), eabo5633.

Publication 2022

DNeasy Blood & Tissue Kit RNeasy Plus Universal Kit Platinum Taq DNA Polymerase High Fidelity Kit TaqMan RNA-to-Ct 1-Step Kit

Blood Cellular Platinum Taq dna polymerase Tissue

Corresponding Organization : Stanford University

Other organizations : Texas Children's Hospital, Baylor College of Medicine, Neurological Research Institute, The University of Texas Medical Branch at Galveston, Massachusetts General Hospital, Howard Hughes Medical Institute

Top 5 similar protocols

Variable analysis

independent variables

DNA/RNA extraction method (DNeasy Blood & Tissue Kit or RNeasy Plus Universal Kit)

dependent variables

Measured PCR/qPCR outcomes

control variables

Platinum Taq DNA Polymerase High Fidelity Kit (for PCR)
TaqMan RNA-to-Ct 1-Step Kit (for qPCR)
Reagents listed in Table 1

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!