Ultrastructural Analysis of BBB Integrity

The brains were harvested two days after surgery. As described previously [9 (link)], sections of the hippocampal CA1 region were prepared for electron microscopy by fixation in 0.5% glutaraldehyde and 1% osmium tetroxide. Samples were dehydrated in acetone solutions at increasing concentrations, embedded in an epoxy resin, and stained with uranyl acetate and lead citrate. The ultrastructural changes of the basal lamina, tight junction and mitochondria, as well as the angioedema surrounding the capillaries all indicative of BBB integrity disruption were observed with JEM-1400 transmission electron microscopy (JEOL, Tokyo, Japan).

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Han D., Li Z., Liu T., Yang N., Li Y., He J., Qian M., Kuang Z., Zhang W., Ni C, & Guo X. (2020). Prebiotics Regulation of Intestinal Microbiota Attenuates Cognitive Dysfunction Induced by Surgery Stimulation in APP/PS1 Mice. Aging and Disease, 11(5), 1029-1045.

Publication 2020

JEM-1400 transmission electron microscopy

Acetone Angioedema Basal lamina Brains Capillaries Citrate Electron microscopy Epoxy resin Glutaraldehyde Hippocampal ca1 region Mitochondria Osmium tetroxide Tight junction Transmission electron microscopy Uranyl acetate

Corresponding Organization : Peking University Third Hospital

Other organizations : National Institutes for Food and Drug Control

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Variable analysis

independent variables

Time after surgery (2 days)

dependent variables

Ultrastructural changes of the basal lamina
Ultrastructural changes of the tight junction
Ultrastructural changes of the mitochondria
Angioedema surrounding the capillaries

control variables

Hippocampal CA1 region
Fixation in 0.5% glutaraldehyde and 1% osmium tetroxide
Dehydration in acetone solutions at increasing concentrations
Embedding in epoxy resin
Staining with uranyl acetate and lead citrate

controls

No positive or negative controls were explicitly mentioned.

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