Cadmium-Induced Cell Viability Assay

Cell viability measurement was performed as previously reported [38 (link)]. Briefly, the rat proximal tubule cells in the logarithmic growth phase in 96-well plates were treated with different concentrations of Cd for 12 h, and this was repeated six times within each group. In addition, blank control wells (with culture medium only) were set up. After Cd treatment, 10 μL of CCK-8 reagent was added to each well, which was shaken and mixed gently and then incubated in the cell incubator in the dark for 1–2 h. OD values were determined using a full-wavelength microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm. The cell viability was calculated according to the formula [(OD treatment group-OD blank group/OD control-OD blank group)] × 100%.