Human NSCLC cell lines and human bronchoalveolar cells were provided by Dr. John Minna (UT Southwestern Medical Center) and cultured as described (27 (link), 28 (link)). A549 and H460 cells expressing vector control or LKB1 were previously described (17 (link)). All cells were mycoplasma free and were identified by DNA fingerprinting. Supplementary Table 1 shows the mutations that they harbor according to COSMIC: Catalogue of Somatic Mutations in Cancer (Cosmic; cancer.sanger.ac.uk). FAK inhibitors PF-562,271 and VS-4718 were obtained from Selleckchem and Verastem, Inc., respectively (29 (link), 30 (link)). Inhibitors were added to mid-log phase cell cultures at the indicated concentrations. All other chemicals were purchased from Sigma-Aldrich.
Protocol detail
Cell lines and inhibitors for NSCLC research
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Corresponding Organization : The University of Texas Southwestern Medical Center
Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, The University of Texas MD Anderson Cancer Center
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Variable analysis
independent variables
- LKB1 expression (vector control or LKB1)
- Not explicitly mentioned
- Mycoplasma-free cell lines
- Cell lines identified by DNA fingerprinting
- FAK inhibitors PF-562,271 and VS-4718 used at indicated concentrations
- Positive control: A549 and H460 cells expressing vector control
- Negative control: A549 and H460 cells expressing LKB1
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