Cell lysates, extracted nuclei and cytoplasm were subjected to western blot analysis, as described previously.21 (link) Briefly, proteins was separated on 10–15% SDS-PAGE gels, and the separated proteins were then transferred to PVDF membranes. The protein blots were blocked with 5% milk and probed sequentially with specific primary antibodies and horseradish peroxidase-conjugated secondary antibodies. The detection of specific proteins on the blots was achieved with enhanced chemiluminescence (Pierce SuperSignal, Thermo Fisher Scientific Inc., Rockford, IL, USA), and the results were captured on X-ray films. Densitometric analysis was performed using the Image-Pro Plus analysis software (Media Cybernetics, Inc., Rockville, MD, USA).