Biomarker Quantification in Cerebrospinal Fluid

CSF samples were obtained by LP following a standard procedure, centrifuged in case of blood contamination, divided into aliquots, and stored in polypropylene tubes at − 80 °C until analysis. CSF total tau (t-tau) and p-tau were measured by chemiluminescent enzyme immunoassays on the automated Lumipulse G600II platform (Fujirebio), as described [28 (link)]. The inter-assay coefficients of variation (CVs) were < 8% for both biomarkers. We used commercially available ELISA kits to measure CSF NfL and 14–3-3 gamma isoform, as described [14 (link), 29 (link)]. SNAP-25 concentrations were determined by running the commercially available SNAP-25 Advantage Kit (Quanterix) on the SIMOA SR-X platform (Quanterix). Ng concentrations were assessed with ELISA technology using the Human Neurogranin (Trunc P75) ELISA Kit (EUROIMMUN). The intra-assay and the inter-assay CVs were < 8% and < 15%, respectively, for all four biomarkers. As previously reported, all CSF samples from patients without autopsy examination, classified as probable sCJD or np-RPD, were tested by second-generation prion CSF RT-QuIC [9 (link)].

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Bentivenga G.M., Baiardi S., Mastrangelo A., Zenesini C., Mammana A., Polischi B., Capellari S, & Parchi P. (2023). Diagnostic and prognostic value of cerebrospinal fluid SNAP-25 and neurogranin in Creutzfeldt-Jakob disease in a clinical setting cohort of rapidly progressive dementias. Alzheimer's Research & Therapy, 15, 150.

Publication 2023

Lumipulse G600II platform SIMOA SR-X platform

14 3 3 Assay Autopsy examination Biomarkers Blood Elisa Enzyme immunoassays Gamma Human Isoform Neurogranin Patients Polypropylene Prion Scjd Snap 25 Trunc

Corresponding Organization :

Other organizations : University of Bologna, Istituto delle Scienze Neurologiche di Bologna

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

CSF total tau (t-tau)
CSF p-tau
CSF NfL
CSF 14-3-3 gamma isoform
CSF SNAP-25 concentrations
CSF Ng concentrations

control variables

CSF samples were obtained by LP following a standard procedure
CSF samples were centrifuged in case of blood contamination
CSF samples were divided into aliquots
CSF samples were stored in polypropylene tubes at − 80 °C until analysis
Inter-assay coefficients of variation (CVs) were < 8% for t-tau and p-tau
Intra-assay and inter-assay CVs were < 8% and < 15%, respectively, for NfL, 14-3-3 gamma isoform, SNAP-25, and Ng

controls

Positive control: CSF samples from patients with probable sCJD or np-RPD were tested by second-generation prion CSF RT-QuIC
Negative control: Not explicitly mentioned

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