HM@WY-Lip/UA were fabricated through the generation of water-in-oil droplets using microfluidics [56 (link)]. The water phase consisted of a 5 wt% mixture of HAMA, WY-Lip/UA, and a photoinitiator (in a ratio of 80:12:8, w/w/w). Additionally, a mixture consisting of paraffin oil and 5 wt% Span 80 was formulated for the oil phase. The oil and water phases were combined and introduced into the microfluidic device through the inlet utilizing a syringe pump, using a flow rate ratio that had been adjusted appropriately. Cross-linking was achieved by exposing the droplets to UV radiation for 5 min. After this, the cross-linked microspheres underwent a cleansing process involving acetone and deionized water, followed by a freeze-drying period of 48 h.
Bright-field microscopy (ZEISS, Germany) was used to examine the morphology and size of HM@WY-Lip/UA. To validate the effective binding of liposomes to HM, liposomes labeled with DiI were used to confirm the successful incorporation of liposomes, which were subsequently observed utilizing LSCM. After 60 s of gold spraying using an ion sputtering instrument, the surface morphology and microstructure of HM or HM@WY-Lip/UA were examined through SEM analysis (FEI Sirion 200, USA). SEM images were obtained at an accelerating voltage of 10 kV.
Bright-field microscopy (ZEISS, Germany) was used to examine the morphology and size of HM@WY-Lip/UA. To validate the effective binding of liposomes to HM, liposomes labeled with DiI were used to confirm the successful incorporation of liposomes, which were subsequently observed utilizing LSCM. After 60 s of gold spraying using an ion sputtering instrument, the surface morphology and microstructure of HM or HM@WY-Lip/UA were examined through SEM analysis (FEI Sirion 200, USA). SEM images were obtained at an accelerating voltage of 10 kV.
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