Arylesterase Activity of Recombinant PON1

The arylesterase activity of recombinant PON1 was measured either in the absence or presence of currant extract at various concentrations (1–10 μg GAE/mL). As the enzyme source, we used 50 µL of cell medium of HEK293 cells transfected, in a 12-well plate, with the pcDNA3.1+/C-(K)-DYK/PON1 vector and cultured in the absence of currant extract, as described above. The arylesterase activity of recombinant PON1 was determined by monitoring the increase in absorbance, following phenyl acetate hydrolysis, at 270 nm for 10 min.

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Lymperopoulos D., Dedemadi A.G., Voulgari M.L., Georgiou E., Dafnis I., Mountaki C., Panagopoulou E.A., Karvelas M., Chiou A., Karathanos V.T, & Chroni A. (2024). Corinthian Currants Promote the Expression of Paraoxonase-1 and Enhance the Antioxidant Status in Serum and Brain of 5xFAD Mouse Model of Alzheimer’s Disease. Biomolecules, 14(4), 426.

Publication 2024

Corresponding Organization :

Other organizations : National Centre of Scientific Research "Demokritos", National and Kapodistrian University of Athens, Harokopio University of Athens

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Variable analysis

independent variables

Concentration of currant extract (1–10 μg GAE/mL)

dependent variables

Arylesterase activity of recombinant PON1

control variables

50 µL of cell medium of HEK293 cells transfected, in a 12-well plate, with the pcDNA3.1+/C-(K)-DYK/PON1 vector and cultured in the absence of currant extract

controls

Arylesterase activity of recombinant PON1 measured in the absence of currant extract (negative control)

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