Metabolite Analysis by CE-MS and HPLC

The dried extracts were dissolved in 20 μL Milli-Q water and analyzed using an Agilent G7100 CE system, an Agilent G6224AA LC/MSD time-of-flight (TOF) system, and an Agilent 1200 series isocratic high-performance liquid chromatography (HPLC) pump equipped with a 1:100 splitter for delivery of the sheath liquid (Agilent Technologies, Palo Alto, CA, USA). Agilent Chem-Station software for CE/MS and MassHunter software for TOFMS were used as the system control and data acquisition software. The CE/MS analytical conditions for cationic and anionic metabolite analyses were as described previously [49 (link)].

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Aikawa S., Nishida A., Hasunuma T., Chang J.S, & Kondo A. (2019). Short-Term Temporal Metabolic Behavior in Halophilic Cyanobacterium Synechococcus sp. Strain PCC 7002 after Salt Shock. Metabolites, 9(12), 297.

Publication 2019

G7100 CE system

Cationic Delivery High performance liquid chromatography

Corresponding Organization : Kobe University

Other organizations : National Cheng Kung University

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Variable analysis

independent variables

Milli-Q water used to dissolve the dried extracts

dependent variables

Metabolite analysis using Agilent G7100 CE system
Metabolite analysis using Agilent G6224AA LC/MSD time-of-flight (TOF) system
Metabolite analysis using Agilent 1200 series isocratic high-performance liquid chromatography (HPLC) pump

control variables

Agilent Chem-Station software for CE/MS and MassHunter software for TOFMS used as the system control and data acquisition software
CE/MS analytical conditions for cationic and anionic metabolite analyses as described previously [49]

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