Protocol detail

Vitamin D, Inflammation, and Immune Biomarkers

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Blood samples were obtained at the first and last study visits and stored at −80°C until determination. 25(OH)D concentrations were assessed by isotope dilution/online solid-phase extraction liquid chromatography/tandem mass spectrometry (ID-XLC-MS/MS) at the Endocrine Laboratory of the Amsterdam University Medical Center (22 (link)). The limit of quantitation was 4.0 nmol/L; intra-assay CV was <6% and interassay CV was <8% for concentrations between 25 and 180 nmol/L. 25(OH)D₂ and 25(OH)D₃ were measured separately. Plasma C-reactive protein (CRP), IL-6, and LL-37 concentrations were measured at the Leiden University Medical Center according to the manufacturer's instructions (CRP and IL-6: Meso Scale Diagnostics; LL-37: Hycult Biotech).

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Rafiq R., Aleva F.E., Schrumpf J.A., Daniels J.M., Bet P.M., Boersma W.G., Bresser P., Spanbroek M., Lips P., van den Broek T.J., Keijser B.J., van der Ven A.J., Hiemstra P.S., den Heijer M, & de Jongh R.T. (2022). Vitamin D supplementation in chronic obstructive pulmonary disease patients with low serum vitamin D: a randomized controlled trial. The American Journal of Clinical Nutrition, 116(2), 491-499.

Publication 2022

LL-37

25 oh d3 Assay Blood C reactive protein Diagnostics Dilution Endocrine Isotope Liquid chromatography Ms ms Plasma Solid phase extraction

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

25(OH)D concentrations
Plasma C-reactive protein (CRP) concentrations
Plasma IL-6 concentrations
Plasma LL-37 concentrations

control variables

Blood samples were obtained at the first and last study visits
Blood samples were stored at −80°C until determination
25(OH)D concentrations were assessed by isotope dilution/online solid-phase extraction liquid chromatography/tandem mass spectrometry (ID-XLC-MS/MS) at the Endocrine Laboratory of the Amsterdam University Medical Center
The limit of quantitation was 4.0 nmol/L
Intra-assay CV was <6% and interassay CV was <8% for concentrations between 25 and 180 nmol/L
25(OH)D2 and 25(OH)D3 were measured separately
Plasma C-reactive protein (CRP), IL-6, and LL-37 concentrations were measured at the Leiden University Medical Center according to the manufacturer's instructions (CRP and IL-6: Meso Scale Diagnostics; LL-37: Hycult Biotech)

controls

None explicitly mentioned

Annotations

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