Tumor Immunology in Mouse Models

GA (Teva Neuroscience), anti-PIR-A/B (6C1, BD Biosciences) and anti-PIR-B (R&D, Santa Cruz). GA-FITC was synthesized by desalting GA using Zeba columns (Pierce) followed by fluorescein isothiocyanate conjugation (Pierce). PHA, anti-Gr-1, anti-CD115, anti-F4/80, anti-CD11b, and isotype-matched antibodies were purchased from eBioscience. MCA26 (23 (link)) and LLC cells (ATCC # CBL-1642) were subcutaneously (sc) implanted in female BALB/c and C57BL/6 mice, respectively. Wild-type mice were obtained from the Jackson Laboratory (Bar Harbor, ME). Colonies of HA-TCR transgenic (Tg) mice (24 ), and PIR-B deficient mice (22 (link)) were established from the mice generously provided by Drs. C. A. Bona (Icahn School of Medicine at Mount Sinai, New York, NY) and T. Takai (Tohoku University, Japan). Animals were handled in accordance with the institutional animal guidelines.

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van der Touw W., Kang K., Luan Y., Ma G., Mai S., Qin L., Bian G., Zhang R., Mungamuri S.K., Hu H.M., Zhang C.C., Aaronson S.A., Feldmann M., Yang W.C., Chen S.H, & Pan P.Y. (2018). Glatiramer acetate enhances myeloid-derived suppressor cell function via recognition of paired immunoglobulin-like receptor-B. Journal of immunology (Baltimore, Md. : 1950), 201(6), 1727-1734.

Publication 2018

PHA anti-Gr-1 anti-CD115 anti-F4/80 anti-CD11b BALB/c C57BL/6 mice Wild-type mice

Animal Anti d Antibodies C57bl mice Cd11b Cells Female Fitc Fluorescein Isothiocyanate Isotype Mice Transgenic mice

Corresponding Organization : Kaohsiung Medical University

Other organizations : Cornell University, Providence Portland Medical Center, The University of Texas Southwestern Medical Center, University of Oxford, Agricultural Biotechnology Research Center, Academia Sinica

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Variable analysis

independent variables

GA (Teva Neuroscience)
Anti-PIR-A/B (6C1, BD Biosciences)
Anti-PIR-B (R&D, Santa Cruz)
Anti-Gr-1
Anti-CD115
Anti-F4/80
Anti-CD11b
Isotype-matched antibodies
MCA26 (23 (link))
LLC cells (ATCC # CBL-1642)
HA-TCR transgenic (Tg) mice (24 (no link found))
PIR-B deficient mice (22 (link))

dependent variables

GA-FITC

control variables

Female BALB/c mice
Female C57BL/6 mice
Wild-type mice from the Jackson Laboratory (Bar Harbor, ME)

positive controls

None specified

negative controls

None specified

Annotations

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