GFP-expressing mouse OSCs (1 × 104) were injected directly into each ovary of wild-type mice at 2 months of age. Between 7–8 months of age, transplanted mice were subjected to an induced ovulation protocol. Cumulus-oocyte complexes were collected from the oviducts and assessed by direct fluorescence microscopy for GFP expression. For in-vitro fertilization (IVF), cumulus-oocyte complexes were mixed wild-type sperm for 4–5 h, washed and transferred to KSOM-AA medium (Irvine Scientific). Light and fluorescence microscopic examination was performed every 24 h for a total of 144 h to monitor embryo development48 (link). Ovarian tissue harvested at the time of oocyte collection was processed for immunohistochemical detection of GFP, as detailed46 (link).
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Transplantation of GFP-expressing Mouse OSCs
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Other organizations : Massachusetts General Hospital, Saitama Medical University, Social Insurance Saitama Chuo Hospital, Harvard University
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Variable analysis
independent variables
- Injection of GFP-expressing mouse OSCs (1 × 10^4) directly into each ovary of wild-type mice at 2 months of age
- GFP expression in cumulus-oocyte complexes collected from the oviducts
- Embryo development monitored by light and fluorescence microscopy every 24 h for a total of 144 h
- Wild-type mice
- Time of induced ovulation (between 7–8 months of age)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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