Purification of alpha-1-antitrypsin from human plasma

Purified human plasma pooled A1AT (Prolastin, Grifols) was used for in vitro experiments. To remove high molecular mass proteins, specifically IgA [16 (link),23 (link),24 (link)] Prolastin was first diluted in phosphate-buffered saline (PBS) and repurified by ultrafiltration using a centricon-100 kDa and centricon-30 kDa cutoff. The IgA-free preparation of A1AT was subjected to quantitative analysis using bicinchoninic acid assay (BCA assay, Pierce BCA Protein Assay Kit, Thermo Scientific) and to qualitative evaluation using Western blot analysis with specific anti-A1AT and anti-IgA antibody.

Free full text: Click here

Koepke J., Dresel M., Schmid S., Greulich T., Beutel B., Schmeck B., Vogelmeier C.F., Janciauskiene S, & Koczulla A.R. (2015). Therapy with Plasma Purified Alpha1-Antitrypsin (Prolastin®) Induces Time-Dependent Changes in Plasma Levels of MMP-9 and MPO. PLoS ONE, 10(1), e0117497.

Publication 2015

Prolastin Pierce BCA Protein Assay Kit

Anti antibody Assay Bicinchoninic acid Human Mass proteins Phosphate Plasma Prolastin Protein Saline Ultrafiltration Western blot analysis

Corresponding Organization :

Other organizations : Philipps University of Marburg, Universities of Giessen and Marburg Lung Center, German Center for Lung Research, Medizinische Hochschule Hannover

Top 5 similar protocols

Variable analysis

independent variables

Dilution of Prolastin in phosphate-buffered saline (PBS)
Repurification of Prolastin by ultrafiltration using a centricon-100 kDa and centricon-30 kDa cutoff

dependent variables

Quantitative analysis of IgA-free A1AT preparation using bicinchoninic acid assay (BCA assay)
Qualitative evaluation of IgA-free A1AT preparation using Western blot analysis with specific anti-A1AT and anti-IgA antibody

control variables

Purified human plasma pooled A1AT (Prolastin, Grifols)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!