Laser Capture Microdissection and miRNA Analysis

Cells were laser captured using the MMI CellCut Plus (Molecular Machines & Industries, Glattbrugg Switzerland) as previously described [52 (link)] and lysed using the lysis buffer RLT from Qiagen Micro RNAeasy Kit (Qiagen, Gaithersburg, MD). For microRNA analysis, total RNA lysates from cells were purified using the miRNeasy Micro Kit (Qiagen, Gaithersburg, MD) in accordance with manufacturer instructions. The mRNA and miRNA data have both been deposited in NCBI's Gene Expression Omnibus (GEO) and are accessible through GEO Series accession number GSE63627 and GSE63633, respectively.

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Castro N.P., Fedorova-Abrams N.D., Merchant A.S., Rangel M.C., Nagaoka T., Karasawa H., Klauzinska M., Hewitt S.M., Biswas K., Sharan S.K, & Salomon D.S. (2014). Cripto-1 as a novel therapeutic target for triple negative breast cancer. Oncotarget, 6(14), 11910-11929.

Publication 2014

Micro RNAeasy Kit miRNeasy Micro Kit

Buffer Cells Gene expression Mirna Mrna

Corresponding Organization :

Other organizations : National Cancer Institute, Center for Cancer Research

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Variable analysis

independent variables

Laser capture using the MMI CellCut Plus

dependent variables

MRNA expression
MicroRNA expression

control variables

Lysis buffer RLT from Qiagen Micro RNAeasy Kit
MiRNeasy Micro Kit (Qiagen, Gaithersburg, MD)

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