The total RNA from samples was extracted using Plant RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) with additional treatment with RNase-Free DNase Set (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and protocols. Evaluation of the quantity and purity of RNA was performed on a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA); integrity was assessed using LabChip GX Touch™ system (PerkinElmer, Waltham, MA, USA).
MACE libraries were constructed using the MACE kit (Zawada et al., 2014 (link)) according to the manual provided with the kit. Sequencing was performed on Illumina NextSeq 500 platform. PCR duplicates were removed automatically.
All raw data are available in NCBI SRA (PRJNA630059 ).
MACE libraries were constructed using the MACE kit (Zawada et al., 2014 (link)) according to the manual provided with the kit. Sequencing was performed on Illumina NextSeq 500 platform. PCR duplicates were removed automatically.
All raw data are available in NCBI SRA (
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