Synthesis of Thiazole-Substituted Enoates

IHSF001 ((E)-ethyl 4-oxo-4-(thiazol-2-ylamino)but-2-enoate) was obtained from AKos Consulting & Solutions GmbH (Klarenbeek, Netherlands). The syntheses of IHSF058-115 were described in Ref. [18 (link)] and those of IHSF133 and compounds 142-144, in the Supplementary Materials. WA was sourced from Santa Cruz Biotechnology (Dallas, TX, USA) and CEL, from Merck (Kenilworth, NJ, USA). All compounds were dissolved in DMSO at 10 mM. phRL-CMV and pCMV-Luc were obtained from Promega (Madison, WI, USA) and Origene (Rockville, MD, USA), respectively. pCMV-β-Gal was previously described [57 (link)]. Adenovirus Ad-CMV-RLUC was obtained from SignaGen Laboratories (Frederick, MD, USA).