Immunohistochemical Analysis of ST6GAL1 in PDAC

Tissue microarrays containing nonmalignant and PDAC patient tissues were obtained from US Biomax Inc. (PA1001c, PA2072a, PA1921, PA1002b). Sections were IHC-stained for ST6GAL1 as reported (25 (link), 26 (link)). Briefly, sections were subjected to antigen retrieval using Antigen Unmasking Solution, Citric Acid Based (Vector Laboratories); blocked with 2.5% horse serum for 1 hour; and incubated overnight at 4°C with goat polyclonal antibody against ST6GAL1 (see Supplemental Table 3 for antibody information). Sections were incubated with ImmPRESS-HRP anti-goat IgG for 1 hour and developed using ImmPACTNovaRED or ImmPACTDAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with Nikon NIS-Elements imaging software.