EBV RNA Enrichment and Sequencing

Sequencing libraries were constructed with SureSelect QXT Target Enrichment kit (Agilent Technologies, G9683B) and custom-designed EBV RNA bait probes (Agilent Technologies, 5190-4806), according to the manufacturer’s instructions. RNA bait probes were designed and kindly shared by Dr. Daniel P. Depledge [11 (link)]. EBV-enriched libraries were sequenced on a NexSeq500 Illumina device, with 300 cycle mid-output (2 × 150 bp) NextSeq Reagent kits v2 (Illumina Corporation, 20024905). FASTQ files of raw sequencing data were deposited in the NCBI database with the Sequence Read Archive (SRA) accession number PRJNA679281 (Supplemental Table S1).