The first urine sample in the morning and the first urine sample after SC were taken from all the subjects. They were collected in polyethylene tubes previously washed with diluted nitric acid and frozen at −80 °C until analyzed, and once the container was handed over, it was measured and codified. Prior to analyses, samples were thawed and homogenized by shaking.
A 10 mL quantity was used to obtain the different evaluated parameters. Specific gravity was analyzed in situ with a precalibrated refractometer (URC-Ne, Atago, Japan), as previously described [32 (link)]. Biochemical variables (pH, microalbuminuria (MA), erythrocytes) were measured by placing a reagent strip (Combur Test, Roche, Spain) in a small portion of urine samples. Next, the strip was placed inside an automatic reflection photometer (Urisys 1100, Roche, Spain) to measure the parameters after a 1 min incubation time.
A 10 mL quantity was used to obtain the different evaluated parameters. Specific gravity was analyzed in situ with a precalibrated refractometer (URC-Ne, Atago, Japan), as previously described [32 (link)]. Biochemical variables (pH, microalbuminuria (MA), erythrocytes) were measured by placing a reagent strip (Combur Test, Roche, Spain) in a small portion of urine samples. Next, the strip was placed inside an automatic reflection photometer (Urisys 1100, Roche, Spain) to measure the parameters after a 1 min incubation time.
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