Mice were anaesthetized with a mixture of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, The Netherlands; 0.4 mg/kg), intraperitoneally (i.p.). EMKA invasive measurement of dynamic resistance (EMKA Technologies, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, The Netherlands; 0–25 mg/mL, 10 % puff for 10 s.) was used to determine the lung function on day 14 [13 (link)]. Basal airway resistance values of each individual mouse as measured prior to methacholine exposure were deducted from the resistance as measured upon methacholine exposure (ΔR; Fig. 1 b–c).
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