RNA was purified from snap-frozen packed C. elegans pellets using TRIzol Reagent as directed by the manufacturer’s instructions51 (link). RNA was resuspended in 50 uL sterile water. RNA concentration and purity were assessed using a NanoPhotometer® NP80 spectrophotometer (Implen GmbH, Munich, Germany). RNA integrity was assessed by 1% Tris/Borate/EDTA (TBE) agarose gel electrophoresis.
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