Piezo-2 Protein Levels in Intracerebral Hemorrhage

Animals were anaesthetized at 8 hours after ICH. An ipsilateral basal ganglia sample was harvested to determine Piezo-2 protein levels (n = 3 per group). Western blotting was performed as described previously^{2 (link)}. Each sample that contained 50 μg protein was separated by SDS-polyacrylamide gel electrophoresis. Proteins were blotted onto a PVDF membrane and incubated with polyclonal rabbit anti-FAM38B (Abcam; 1:500 dilution) and polyclonal rabbit anti-β-actin (Proteintech; 1:1000 dilution). The secondary antibody was goat anti-rabbit IgG (Beyotime; 1:1000 dilution). The antigen-antibody complexes were visualized by the ECL electrochemiluminescence and quantified by Quantity-one. Representative strips were proceeded through “Microsoft Office PowerPoint 2016”.

Free full text: Click here

Gong Y., Gong Y., Hou Z., Guo T., Deng J., Hao S, & Wang B. (2019). Establishment of an Experimental Intracerebral Haemorrhage Model for Mass Effect Research using a Thermo-sensitive Hydrogel. Scientific Reports, 9, 13838.

Publication 2019

goat anti-rabbit IgG

Actin Animals Anti igg Antibody Antigen antibody complexes Basal ganglia Dilution Goat Membrane Proteins Pvdf Rabbit Sds polyacrylamide gel electrophoresis

Corresponding Organization :

Other organizations : Chongqing University, Dalian Medical University, Chongqing Medical University, Second Affiliated Hospital of Chongqing Medical University, Chongqing Technology and Business University

Top 5 similar protocols

Variable analysis

independent variables

Time after ICH when animals were anaesthetized (8 hours)

dependent variables

Piezo-2 protein levels in the ipsilateral basal ganglia sample

control variables

Sample size (n = 3 per group)
Protein amount (50 μg per sample)
Primary antibodies (polyclonal rabbit anti-FAM38B and polyclonal rabbit anti-β-actin)
Secondary antibody (goat anti-rabbit IgG)
Detection method (ECL electrochemiluminescence)
Quantification method (Quantity-one)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!