Estradiol and Hypoxia Pathway Inhibitors

17β-Estradiol (E₂), cobalt chloride (CoCl₂) (Sigma Aldrich, St. Louis, MO, USA)). Mocetinostat, GSK-126, and GSK-J4 inhibitors were as in the literature [49 (link),55 (link),56 (link)]. Antibody to β4 integrin (450-11A) was as in the work of [71 (link)]. Antibodies: E-cadherin (GeneTex, Irvine, CA, USA; GTX100443), ERβ (L-20, Santa Cruz Biotechnology, Dallas, TX, USA; SC-6822, CWK-F12, DSHB), eNOS (BD Biosciences, Franklin Lakes, NJ, USA; BD610297), EZH2 (D2C9, Cell Signaling, Danvers, MA, USA; #5246s), H3K27me3 (Active Motif, Carlsbad, CA, USA; #39156 and #39155), HIF-2α (Novus Biologicals, Centennial, CO, USA; NB100-132), HIF-1α (Novus Biological NB100-105), N-cadherin (Thermo-Fisher, Waltham, MA, USA; #PA5-19486), integrin β3 (Genetex, Irvine, CA, USA; GTX111672), Snail (Genetex GTX125918), Vimentin (Bethyl, Montgomery, TX, USA; A301-620A-T), Laminin beta 1 (Abcam, Cambridge, UK; ab108536), Fibrillarin (Thermo Fisher MA3-16771), HSP90 (StressMarq, Victoria, BC, Canada; SPC-104), IgG (Normal Rabbit, Millipore, Burlington, MA, USA #12-370, Normal Rabbit, Bethyl #S120-101), Tubulin (DM1A, Abcam #ab7291), β Actin (Abcam).