TUNEL staining protocol was according to a Roche protocol as previously described [45 (link), 64 –66 ]. Tunel staining was also incubated with anti-TH (1:250; Merck-Millipore) and FITC-conjugated anti-mouse Alexa Fluor-488 antibody (1:2000 v/v Molecular Probes, UK) for 1 h at 37 °C and then observed with Leica DM6 (Milan, Italy) associated with an Imaging system (LasX Navigator, Milan, Italy).
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