HMGA1 and FoxO1 Protein Analysis

A polyclonal-specific antibody against HMGA1^{23 (link)} was used to analyze HMGA1 protein expression in HEK-293 and HepG2 cells, and in liver nuclear extracts from normal and Hmga1 mutant mice. An anti-FoxO1 antibody (Santa Cruz Biotechnology) was employed to measure total FoxO1 in both human cell lines and animal tissues. An anti phospho-FoxO1 (Thr24) (Cell Signaling Technology) was used to analyze the levels of pFoxO1 protein in both nuclear and cytoplasmatic extracts.

Free full text: Click here

Chiefari E., Arcidiacono B., Palmieri C., Corigliano D.M., Morittu V.M., Britti D., Armoni M., Foti D.P, & Brunetti A. (2018). Cross-talk among HMGA1 and FoxO1 in control of nuclear insulin signaling. Scientific Reports, 8, 8540.

Publication 2018

anti-FoxO1 antibody

Animal Anti antibody Antibody Cell lines Cytoplasmatic Hepg2 cells Hmga1 Human Liver extracts Mice Protein Tissues

Corresponding Organization :

Other organizations : Magna Graecia University, Rambam Health Care Campus

Top 5 similar protocols

Variable analysis

independent variables

Hmga1 mutant mice

dependent variables

HMGA1 protein expression
Total FoxO1 protein levels
Phospho-FoxO1 (Thr24) protein levels

control variables

HEK-293 cells
HepG2 cells
Normal mice liver nuclear extracts

controls

Polyclonal-specific antibody against HMGA1
Anti-FoxO1 antibody (Santa Cruz Biotechnology)
Anti phospho-FoxO1 (Thr24) antibody (Cell Signaling Technology)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!