Tumor necrosis factor (TNF)-α (300-01A) was purchased from PeproTech (Rocky Hill, NJ, USA). Resveratrol (SML0963) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Endothelial cell medium (ECM), fetal bovine serum (FBS), endothelial cell growth supplement (ECGS), and penicillin/streptomycin solution (P/S) (1001) were purchased from Sciencell (CA, USA). Fluorescein (FITC)-conjugated affiniPure Goat Anti-Rabbit IgG (H+L) (111-095-144) was purchased from Jackson ImmunoResearch Company (USA). Rabbit Anti-Factor VIII related antigen (BA0046) was purchased from Boster (China). SIRT1 (ab32441), SIRT2 (ab51023), SIRT3 (ab86671), SIRT4 (ab105039), SIRT5 (ab105040), SIRT6 (ab62739), SIRT7 (ab105038) antibodies, and BCA protein assay kit (ab102536) were provided by Abcam (Abcam, USA) [11 (link)].
Protocol detail
Resveratrol Modulates SIRT Expression
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Anti igg
Antibodies
Antigen
Assay
Endothelial cell
Factor viii
Fetal bovine serum
Fitc
Fluorescein
Goat
Penicillin
Protein
Rabbit
Resveratrol
Sirt1
Sirt2
Sirt3
Sirt6
Streptomycin
Supplement
Tumor necrosis factor α
Corresponding Organization :
Other organizations : Fujian Medical University, Fujian Provincial Hospital
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Variable analysis
independent variables
- Tumor necrosis factor (TNF)-α
- Resveratrol
- Not explicitly mentioned
- Endothelial cell medium (ECM)
- Fetal bovine serum (FBS)
- Endothelial cell growth supplement (ECGS)
- Penicillin/streptomycin solution (P/S)
- Fluorescein (FITC)-conjugated affiniPure Goat Anti-Rabbit IgG (H+L)
- Rabbit Anti-Factor VIII related antigen
- SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, SIRT7 antibodies
- BCA protein assay kit
- Not explicitly mentioned
- Not explicitly mentioned
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