Protocol detail

Multi-Site MALDI-TOF Sickle Cell Screening

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All samples were analyzed at two MALDI-TOF facilities (the University of Burgundy’s CLIPP facility (Dijon, France) and Lille University Hospital’s neonatal screening laboratory (Lille, France)), according to the previously described procedure [11 (link)]. At both facilities, samples were prepared for MS measurements using a research version of the NeoSickle^® kit (Biomaneo). Samples were deposited in quadruplicate on a 384-spot polished steel MALDI target (Bruker Daltonik GmbH). At both facilities, MS was performed with a MALDI-TOF system: An AutoFlex™ Speed with a 2000 Hz Smartbeam™ II laser (Bruker Daltonik GmbH) in Dijon, and an AutoFlex™ III with a 200 Hz Smartbeam^™ laser (Bruker Daltonik GmbH) in Lille.

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El Osta M., Naubourg P., Grunewald O., Renom G., Ducoroy P, & Périni J.M. (2019). The Reliable, Automatic Classification of Neonates in First-Tier MALDI-MS Screening for Sickle Cell Disease. International Journal of Neonatal Screening, 5(3), 31.

Publication 2019

MALDI target AutoFlex™ Speed Smartbeam™ II laser AutoFlex™ III

Maldi Steel

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Variable analysis

independent variables

MALDI-TOF facilities (the University of Burgundy's CLIPP facility (Dijon, France) and Lille University Hospital's neonatal screening laboratory (Lille, France))
Research version of the NeoSickle® kit (Biomaneo) used for sample preparation

dependent variables

Mass spectrometry (MS) measurements

control variables

Sample preparation procedure, as previously described [11]
MALDI-TOF systems used: AutoFlex™ Speed with a 2000 Hz Smartbeam™ II laser (Bruker Daltonik GmbH) in Dijon, and AutoFlex™ III with a 200 Hz Smartbeam™ laser (Bruker Daltonik GmbH) in Lille

controls

No positive or negative controls were explicitly mentioned in the provided information.

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