RiboBio (Guangzhou, China) was commissioned to chemically synthesize biotinylated miR-503-5p. Biotinylation mutants and biotinylation NC were used as controls, and the mutated sequence was 3′-GACGUCUUGACAAGGGGCAGGUU-5′. The cells were transfected with these biotinylated oligonucleotides and maintained with M-280 streptavidin magnetic beads (Invitrogen, USA) to obtain the bound RNA,23 (link) and then qRT‒PCR was used to measure the level of ZSCAN16-AS1 in bound RNA.
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Biotinylated miR-503-5p Capture and Analysis
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Corresponding Organization :
Other organizations : Bengbu Medical College, Affiliated Hospital of Jiangsu University, Jiangsu University
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Variable analysis
independent variables
- Biotinylated miR-503-5p
- Biotinylation mutants
- Biotinylation NC
- Level of ZSCAN16-AS1 in bound RNA
- M-280 streptavidin magnetic beads
- Biotinylation mutants as positive control
- Biotinylation NC as negative control
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