Stereological Analysis of Pancreatic Cells

The Computer Assisted Stereology Toolbox (CAST) 2.0 system (Olympus, Ballerup, Denmark) was used to perform all the microscopic measurements of beta, alpha and delta cell and amyloid deposit volume using the stereology fundaments previously described and validated [31 (link), 33 (link)–35 (link)]. The person who performed the microscopic measurements (RGM) was blinded to the metabolic status of each baboon and the reproducibility of the CAST measurements was estimated twice in five specimens with a coefficient of variation less than 5%.

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Guardado-Mendoza R., Perego C., Finzi G., La Rosa S., Capella C., Jimenez-Ceja L.M., Velloso L.A., Saad M.J., Sessa F., Bertuzzi F., Moretti S., Dick EJ J.r., Davalli A.M, & Folli F. (2015). Delta cell death in the islet of Langerhans and the progression from normal glucose tolerance to type 2 diabetes in non-human primates (Baboons, Papio hamadryas). Diabetologia, 58(8), 1814-1826.

Publication 2015

CAST) 2.0 system

Amyloid deposit Baboon Delta cell Microscopic

Corresponding Organization :

Other organizations : The University of Texas Health Science Center at San Antonio, University of Milan, University of Insubria, Universidad de Guanajuato, Universidade Estadual de Campinas (UNICAMP), Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda, Texas Biomedical Research Institute, IRCCS Ospedale San Raffaele

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Beta cell volume
Alpha cell volume
Delta cell volume
Amyloid deposit volume

control variables

Metabolic status of each baboon (blinded)
Reproducibility of CAST measurements (coefficient of variation less than 5%)

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