At first, each of the samples was cultured in the Lowenstein-Johnson medium, then after the growth, 3 distinct colonies from each sample were subcultured individually in the Lowenstein-Johnson medium to expand further growth. BDQ was purchased from Sigma-Aldrich (St. Louis, MO, USA). The objective of this subculturing process was to assess BDQ resistance using the Microplate Alamar Blue Assay (MABA) method. Alamar Blue was purchased from AbD Serotec (Oxford, UK).
Madadi-Goli N., Ahmadi K., Kamakoli M.K., Azizi M., Khanipour S., Dizaji S.P., Nasehi M., Siadat S.D., Fateh A, & Vaziri F. (2024). The importance of heteroresistance and efflux pumps in bedaquiline-resistant Mycobacterium tuberculosis isolates from Iran. Annals of Clinical Microbiology and Antimicrobials, 23, 36.
Other organizations :
Pasteur Institute of Iran, Kermanshah University of Medical Sciences, Iran University of Medical Sciences, Ministry of Health and Medical Education
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