Antibody Generation and Characterization for Jacob

Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285–299 and 400–314, Jb150) have been described previously [14 (link), 21 (link), 22 (link)]. The following commercial primary antibodies were used in the present study: mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403), anti-MAP2, Alexa Fluor 488 conjugated (Merck, MAB 3418x), mouse monoclonal anti-LaminB1 (ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova) and anti-rabbit IgG (Dianova).

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Samer S., Raman R., Laube G., Kreutz M.R, & Karpova A. (2021). The nuclear lamina is a hub for the nuclear function of Jacob. Molecular Brain, 14, 9.

Publication 2021

mouse monoclonal anti-MBP mouse monoclonal anti-GFP peroxidase-conjugated anti-mouse IgG

Alexa fluor 488 Amino acid Anti antibodies Anti igg Antibodies Map2 Mouse Peroxidase Rabbit

Corresponding Organization :

Other organizations : Leibniz Institute for Neurobiology, Center for Behavioral Brain Sciences

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Variable analysis

independent variables

Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285–299 and 400–314, Jb150)

dependent variables

Not explicitly mentioned

control variables

Commercial primary antibodies: mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403), anti-MAP2, Alexa Fluor 488 conjugated (Merck, MAB 3418x), mouse monoclonal anti-LaminB1 (ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN)

controls

Positive controls not specified
Negative controls not specified

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