Isolation and Characterization of Exosomes from CAFs

CAFs were cultured under both normoxic (CAFs-N) and hypoxic (CAFs-H) conditions. Exosomes were purified from CAFs-N (CAFs-N-Exo) or CAFs-H (CAFs-H-Exo) via ultracentrifugation, as previously described (26 (link)). Exosomes were then resuspended and stored immediately at −80°C. For NTA, the size distribution of exosomes was determined using a ZetaView nanoparticle tracking analyzer (Particle Metrix). For transmission electron microscopy (TEM), the exosomes were fixed, loaded, and stained with 1% phosphotungstic acid. The morphology of the exosomes was then observed using a transmission electron microscope.

Free full text: Click here

Yuan H., Chen B., Chai R., Gong W., Wan Z., Zheng B., Hu X., Guo Y., Gao S., Dai Q., Yu P, & Tu S. (2022). Loss of exosomal micro-RNA-200b-3p from hypoxia cancer-associated fibroblasts reduces sensitivity to 5-flourouracil in colorectal cancer through targeting high-mobility group box 3. Frontiers in Oncology, 12, 920131.

Publication 2022

ZetaView nanoparticle tracking analyzer

Cafs Exosomes H conditions Hypoxic Phosphotungstic acid Transmission electron microscope Ultracentrifugation

Corresponding Organization : Zhejiang Provincial People's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Culture conditions (normoxic (CAFs-N) or hypoxic (CAFs-H))

dependent variables

Exosome size distribution (determined by nanoparticle tracking analysis)
Exosome morphology (observed by transmission electron microscopy)

control variables

Exosome purification method (ultracentrifugation, as previously described)
Exosome storage conditions (-80°C)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!